Microbiology Unit 1 Test Review Quizlet What Are the Parts of the Atom
Microbiology MCQ – 01
(Basics of Microbiology – Part one)
1. The process in which all living cells, spores and viruses are completely destroyed from an object is called _____.
a. Disinfection
b. Pasteurization
c. Sterilization
d. Antisepsis
ii. UHT sterilization involves high temperature exposure of objects for _____.
a. ane – 3 seconds
b. 1 – three minutes
c. one – 3 hours
d. 10 – 30 hours
3. _______ is an antiseptic.
a. Chlorine
b. Copper Sulphate
c. Ozone
d. 70% ethanol
4. Mistake rate of Taq Deoxyribonucleic acid polymerase is _____
a. 10-iv
b. 10-xiv
c. 10-24
d. 10-40
5. The best grade of Dna to transform E. coli is ______.
a. Linear
b. Covalently closed
c. Nicked circles
d. Nicked linear
six. ______ phage is widely used for phage display technique.
a. M13
b. Φ6
c. λ phage
d. T7 phage
7. _____ RNA polymerase has a single subunit.
a. East. coli
b. Bacillus subtilis
c. T7
d. λ phage
8. Aforementioned Dna sequence may lawmaking for more than one poly peptide by ______
a. Gene splitting
b. RNA splitting
c. Alternative splicing of RNA
d. None
9. Bacterial cells can be stained with ____ to reveal the presence of lipid inclusions.
a. Saffranin
b. Methylene blue
c. Trypan blueish
d. Sudan dyes
10. Glucose that is taken in by a bacterial cell is made to stay within the cell past _______.
a. Attaching to membranes
b. Immediate utilization
c. Phosphorylation
d. Conversion to Fructose
11. Magnetosomes are inclusion of _____ in certain bacteria
a. Iron oxide
b. Iron phosphate
c. Iron nitrates
d. Iron chlorides
12. In a population of 1 Ten 10^viii bacterial cells, if there is fifty% killing ______ number of cells would remain viable.
a. 0.five Ten 108
b. 1 X 104
c. 50
d. 0.ii X 108
13. _____ is a predator bacterium that infects other gram negative bacteria.
a. Haemophilus influenza
b. Pseudomonas putida
c. Proteus mirabilis
d. Bdellovibrio
14. ______ are prokaryotic, but have the power to comport out oxygenic photosynthesis.
a. Photorhizobium
b. Blue-green alga
c. Chlorobium
d. Rhodospirillum
15. ______ is the only bacterial genus where sterols are nowadays in the jail cell membrane.
a. Escherichia
b. Mycoplasma
c. Chlamydea
d. Vibrio
More Microbiology MCQs
Answer Cardinal and Explanations
1. Ans. (c). Sterilization
Sterilization: Removal of all microorganism irrespective of harmful or benign from an object. Reagents used usually not specific to microorganisms.
Disinfection: Removal of pathogenic or harmful microbes from the surface of an object by using specific reagents.
Pasteurization: A fractional sterilization process of a liquid by treating the liquid for specific temperature for specific fourth dimension interval which destroys the harmful microbes from the liquid without altering the chemical composition.
Antisepsis: killing pathogenic or harmful microbes by antiseptics
2. Ans. (a). ane – three seconds
UHT: Ultra Loftier Temperature Treatment, A sterilization method
UHT is a method of sterilization of nutrient materials past heating at a temperature of 135oC (270oF) for 1 – three seconds. At this temperature the microbial spores in the food materials is eliminated. UHT treatment is normally used in the pasteurization of milk.
iii. Ans. (d). 70% ethanol
Antiseptic: an anti-microbial amanuensis that applied to skin to preclude or reduced the possible infection
Commonly used antiseptic agents: seventy% alcohol, Hydrogen peroxide, Iodine solution, Polyhexamide and sodium bicarbonate
4. Ans. (a). x-iv
Fault charge per unit of Taq Dna Polymerase: 1 in about 10000 nucleotides (1/10000). The high error rate is because of the fact that Taq DNA polymerase lack the 3′ to 5′ exonuclease proofreading activity.
v. Ans. (b). Covalently closed
6. Ans. (a). M13
Phage display technique: a method to study poly peptide-protein or protein-DNA interactions.
Other phages used for phage display technique: T4, T7 phage, λ phage.
7. Ans. (c). T7 bacteriophage
T7 bacteriophage is highly promoter specific. It transcribe but downstream to T7 promoter.
viii. Ans. (c). Alternative splicing of RNA
Alternate splicing: also chosen differential splicing.
9. Ans. (d). Sudan dyes
ten. Ans. (c). Phosphorylation
Phosphorylated glucose is no recognized by the glucose transport arrangement in the cells and thus they stay in the prison cell.
xi. Ans. (a). Iron oxide
12. Ans. (a). 0.5 10 108
thirteen. Ans. (d). Bdellovibrio
xiv. Ans. (b). Blue-green alga
15. Ans. (b). Mycoplasma
Mycoplasma lacks jail cell wall around the plasma membrane.
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